Pfarr, Nicole and Kirchner, Martina and Lehmann, Ulrich and Leichsenring, Jonas and Merkelbach-Bruse, Sabine and Glade, Julia and Hummel, Michael and Stoegbauer, Fabian and Lehmann, Annika and Trautmann, Marcel and Kumbrink, Joerg and Jung, Andreas and Dietmaier, Wolfgang and Endris, Volker and Kazdal, Daniel and Evert, Matthias and Horst, David and Kreipe, Hans and Kirchner, Thomas and Wardelmann, Eva and Lassen, Ulrik and Buettner, Reinhard and Weichert, Wilko and Dietel, Manfred and Schirmacher, Peter and Stenzinger, Albrecht (2020) Testing NTRK testing: Wet-lab and in silico comparison of RNA-based targeted sequencing assays. GENES CHROMOSOMES & CANCER, 59 (3). pp. 178-188. ISSN 1045-2257, 1098-2264
Full text not available from this repository. (Request a copy)Abstract
NTRK fusions involving three neurotrophic tyrosine receptor kinase genes NTRK1, NTRK2, and NTRK3 and a variety of fusion partners were identified as oncogenic drivers across many cancer types. Drugs that target the chimeric protein product require the identification of the underlying gene fusion. This advocates the diagnostic use of molecular assays ranging from fluorescence in situ hybridization (FISH) and reverse transcription polymerase chain reaction (RT-PCR)/Sanger approaches to targeted next-generation sequencing (NGS). Immunohistochemistry may be used as a screening tool and adjunct diagnostic assay in this context. Although FISH and RT-PCR/Sanger approaches are widely adopted in routine diagnostics, current experience with targeted RNA-based NGS is limited. Here, we report on the analysis of major assays (TruSight TST170 and TruSight RNA Fusion [Illumina]; Archer FusionPlex Solid Tumor, Archer FusionPlex Lung, and Archer FusionPlex Oncology [Archer]; Oncomine Comprehensive Assay v3 RNA and Oncomine Focus RNA [Thermo Fisher Scientific]) that are commercially available. The data set includes performance results of a multicentric comparative wet-lab study as well as an in silico analysis on the ability to detect the broad range of NTRK fusions reported until now. A test algorithm that reflects assay methodology is provided. This data will support implementation of targeted RNA sequencing in routine diagnostics and inform screening and testing strategies that have been brought forward.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FUSION; GENE; FFPE; gene fusion; NGS; NTRK; RNA sequencing |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 Mar 2020 11:19 |
| Last Modified: | 19 Mar 2020 11:19 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25825 |
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