Grevenstein, David and Heilig, Juliane and Dargel, Jens and Oppermann, Johannes and Eysel, Peer and Brochhausen, Christoph and Niehoff, Anja (2020) COMP in the Infrapatellar Fat Pad-Results of a Prospective Histological, Immunohistological, and Biochemical Case-Control Study. JOURNAL OF ORTHOPAEDIC RESEARCH, 38 (4). pp. 747-758. ISSN 0736-0266, 1554-527X
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Knee osteoarthritis (OA) involves several structures and molecules in the joint, which interact in a pathophysiological process. One of these molecules is the cartilage oligomeric matrix protein (COMP). Elevated COMP levels in the synovial fluid as well as in the serum have been described in OA patients. However, this has not been described in the infrapatellar fat pad (IPFP) tissue before. In this prospective trial, we collected 14 IPFPs from patients with high-grade OA (mean age 63.8 +/- 17.6 years) who underwent total knee replacement (OA group) and from 11 healthy patients (mean age 33.7 +/- 14.8 years) who underwent anterior cruciate ligament reconstruction (control group). The presence of macrophages (CD68 and CD206) and proinflammatory cytokines (interleukin 1 beta [IL-1 beta] and IL-6) was analyzed. Histological and immunohistological examinations as well as immunoblotting analysis for COMP, leptin, and matrix-metalloproteinase-3 were performed. The IPFPs of both the OA and control group consisted of adipose tissue and fibrous tissue, and the fibrous tissue showed higher score values than the adipose tissue for COMP staining (intensity as well as stained area) in both groups. Although COMP could be detected in most samples, leptin expression was found only in single specimens. COMP could be detected mostly in the fibrous tissue portion of the IPFP. We speculate that it is involved in a remodeling process taking place in the IPFP during OA. Presence of leptin was irregular in immunohistology, and the control group showed higher scores in case of presence. Interestingly, immunoblotting could detect leptin in all analyzed samples. (c) 2019 The Authors. Journal of Orthopaedic Research (R) published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OLIGOMERIC MATRIX PROTEIN; MESENCHYMAL STEM-CELLS; KNEE OSTEOARTHRITIS; ADIPOSE-TISSUE; SERUM MARKERS; COLLAGEN-II; CARTILAGE; LEPTIN; BONE; INFLAMMATION; osteoarthritis; knee; cartilage; infrapatellar fat pad; Hoffa's fat pad |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Petra Gürster |
| Date Deposited: | 07 Apr 2020 08:33 |
| Last Modified: | 07 Apr 2020 08:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25827 |
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