Santel, Thore and Pflug, Gabi and Hemdan, Nasr Y. A. and Schaefer, Angelika and Hollenbach, Marcus and Buchold, Martin and Hintersdorf, Anja and Lindner, Inge and Otto, Andreas and Bigl, Marina and Oerlecke, Ilka and Hutschenreuter, Antje and Sack, Ulrich and Huse, Klaus and Groth, Marco and Birkemeyer, Claudia and Schellenberger, Wolfgang and Gebhardt, Rolf and Platzer, Mathias and Weiss, Thomas and Vijayalakshmi, Mookambeswaran A. and Krueger, Monika and Birkenmeier, Gerd (2008) Curcumin Inhibits Glyoxalase 1-A Possible Link to Its Anti-Inflammatory and Anti-Tumor Activity. PLOS ONE, 3 (10): e3508. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Background: Glyoxalases (Glo1 and Glo2) are involved in the glycolytic pathway by detoxifying the reactive methylglyoxal (MGO) into D-lactate in a two-step reaction using glutathione (GSH) as cofactor. Inhibitors of glyoxalases are considered as anti-inflammatory and anti-carcinogenic agents. The recent finding that various polyphenols modulate Glo1 activity has prompted us to assess curcumin's potency as an Glo1 inhibitor. Methodology/Principal Findings: Cultures of whole blood cells and tumor cell lines (PC-3, JIM-1, MDA-MD 231 and 1321N1) were set up to investigate the effect of selected polyphenols, including curcumin, on the LPS-induced cytokine production (cytometric bead-based array), cell proliferation (WST-1 assay), cytosolic Glo1 and Glo2 enzymatic activity, apoptosis/necrosis (annexin V-FITC/propidium iodide staining; flow cytometric analysis) as well as GSH and ATP content. Results of enzyme kinetics revealed that curcumin, compared to the polyphenols quercetin, myricetin, kaempferol, luteolin and rutin, elicited a stronger competitive inhibitory effect on Glo1 (K-i = 5.1 +/- 1.4 mu M). Applying a whole blood assay, IC50 values of pro-inflammatory cytokine release (TNF-alpha, IL-6, IL-8, IL-1 beta) were found to be positively correlated with the K-i-values of the aforementioned polyphenols. Moreover, whereas curcumin was found to hamper the growth of breast cancer (JIMT-1, MDA-MB-231), prostate cancer PC-3 and brain astrocytoma 1321N1 cells, no effect on growth or vitality of human primary hepatocytes was elucidated. Curcumin decreased D-lactate release by tumor cells, another clue for inhibition of intracellular Glo1. Conclusions/Significance: The results described herein provide new insights into curcumin's biological activities as they indicate that inhibition of Glo1 by curcumin may result in non-tolerable levels of MGO and GSH, which, in turn, modulate various metabolic cellular pathways including depletion of cellular ATP and GSH content. This may account for curcumin's potency as an anti-inflammatory and anti-tumor agent. The findings support the use of curcumin as a potential therapeutic agent.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kinder- und Jugendmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Oct 2020 09:55 |
| Last Modified: | 21 Oct 2020 09:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30168 |
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