Heijnen, I. A. F. M. and Barnett, D. and Arroz, M. J. and Barry, S. M. and Bonneville, M. and Brando, B. and D'hautcourt, J. L. and Kern, F. and Totterman, T. H. and Marijt, E. W. A. and Bossy, D. and Preijers, F. W. M. B. and Rothe, Gregor and Gratama, J. W. (2004) Enumeration of antigen-specific CD8(+) T lymphocytes by single-platform, HLA tetramer-based flow cytometry: A European multicenter evaluation. CYTOMETRY PART B-CLINICAL CYTOMETRY, 62B (1). pp. 1-13. ISSN 1552-4949, 1552-4957
Full text not available from this repository. (Request a copy)Abstract
Background: HLA class I peptide tetramers represent powerful diagnostic tools for detection and monitoring of antigen-specific CD8(+) T cells. The impetus for the current multicenter study is the critical need to standardize tetramer flow cytometry if it is to be implemented as a routine diagnostic assay. Hence, the European Working Group on Clinical Cell Analysis set out to develop and evaluate a single-platform tetramer-based method that used cytomegalovirus (CMV) as the antigenic model. Methods: Absolute numbers of CMV-specific CD8(+) T cells were obtained by combining the percentage of tetramer-binding cells with the absolute CD8(+) T-cell count. Six send-outs of stabilized blood from healthy individuals or CMV-carrying donors with CMV-specific CD8(+) T-cell counts of 3 to 10 cells/mul were distributed to 7 to 16 clinical sites. These sites were requested to enumerate CD8(+) T cells and, in the case of CMV-positive donors, CMV-specific subsets on three separate occasions using the standard method.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | STEM-CELL TRANSPLANTATION; DIRECT VISUALIZATION; HEALTHY-INDIVIDUALS; IDENTIFY PATIENTS; ELISPOT ASSAY; CLASS-I; CYTOMEGALOVIRUS; PEPTIDE; CD4(+); QUANTIFICATION; tetramer; flow cytometry; CD8 T cells; multicenter evaluation; standardization |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Jun 2021 13:58 |
| Last Modified: | 22 Jun 2021 13:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/36993 |
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