Spagnuolo, G. and Mauro, C. and Leonardi, A. and Santillo, M. and Paterno, R. and Schweikl, Helmut and Avvedimento, E. V. and Rengo, S. (2004) NF-kappa B protection against apoptosis induced by HEMA. JOURNAL OF DENTAL RESEARCH, 83 (11). pp. 837-842. ISSN 0022-0345, 1544-0591
Full text not available from this repository. (Request a copy)Abstract
The cytotoxicity of dental monomers has been widely investigated, but the underlying mechanisms have not been elucidated. We studied the molecular mechanisms involved in cell death induced by HEMA. In human primary fibroblasts, HEMA induced a dose-dependent apoptosis that was confirmed by the activation of caspases-8, -9, and -3. We found an increase of reactive oxygen species (ROS) and NF-kappaB activation after HEMA exposure. Blocking of ROS production by antioxidants had no direct influence on apoptosis caused by HEMA, but inhibition of NF-kappaB increased the fraction of apoptotic cells. Accordingly, mouse embryonic fibroblasts (MEF) from p65-/- mice were more susceptible to HEMA-induced apoptosis than were wild-type controls. Our results indicate that exposure to HEMA triggers apoptosis and that this mechanism is not directly dependent upon redox signaling. Nevertheless, ROS induction by HEMA activates NF-kappaB, which exerts a protective role in counteracting apoptosis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN GINGIVAL FIBROBLASTS; IN-VITRO; ENDOTHELIAL-CELLS; ACTIVATION; EXPRESSION; COMPONENTS; INHIBITORS; INDUCTION; CASPASES; RESINS; NF-kappa B; HEMA; apoptosis; ROS; human fibroblasts |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Prof. Dr. rer. nat. Helmut Schweikl |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Jun 2021 06:46 |
| Last Modified: | 23 Jun 2021 06:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/37001 |
Actions (login required)
 |
View Item |
