Schaeferling, Michael and Wu, Meng and Enderlein, Joerg and Bauer, Henrik and Wolfbeis, Otto S. (2003) Time-resolved luminescence imaging of hydrogen peroxide using sensor membranes in a microwell format. APPLIED SPECTROSCOPY, 57 (11). pp. 1386-1392. ISSN 0003-7028, 1943-3530
Full text not available from this repository. (Request a copy)Abstract
We demonstrate an optical imaging scheme for hydrogen peroxide in a microwell-based format using the europium(III) tetracycline complex as the fluorescent probe, which is incorporated into a poly-acrylonitrile-co-polyacrylamide polymer matrix. The resulting sensor membranes are integrated into a 96-microwell plate. Hydrogen peroxide can be visualized by means of time-resolved luminescence lifetime imaging. The imaging system consists of a fast, gated charge-coupled device (CCD) camera and a pulsed array of 96 light emitting diodes (LEDs). Fluorescence lifetime images are acquired in different modes (rapid lifetime determination, RLD, and phase delay rationing, PDR) and compared with conventional intensity-based methods with respect to sensitivity and the dynamic range of the sensor. The lowest limits of detection can be achieved by the RLD method. The response time of the sensor is comparatively high, typically in the range of 10 to 20 minutes, but the response is reversible. The largest signal changes are observed at pH values between 6.5 and 7.5.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OPTICAL SENSORS; OXYGEN; PH; MICROSCOPY; optical sensor; time-resolved fluorescence imaging; europium tetracycline |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Jul 2021 11:51 |
| Last Modified: | 27 Jul 2021 11:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38426 |
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