Luethgens, Eike and Herrig, Alexander and Kastl, Katja and Steinem, Claudia and Reiss, Bjoern and Wegener, Joachim and Pignataro, Bruno and Janshoff, Andreas (2003) Adhesion of liposomes: a quartz crystal microbalance study. MEASUREMENT SCIENCE AND TECHNOLOGY, 14 (11). pp. 1865-1875. ISSN 0957-0233, 1361-6501
Full text not available from this repository. (Request a copy)Abstract
Three different systems are presented, exploring the adhesion of liposomes mediated by electrostatic and lipid-protein interactions as well as molecular recognition of ligand receptor pairs. Liposomes are frequently used to gain insight into the complicated processes involving adhesion and subsequent events such as fusion and fission mainly triggered by specific proteins. We combined liposome technology with the quartz crystal microbalance (QCM) technique as a powerful tool to study the hidden interface between the membrane and functionalized surface. Electrostatic attraction and molecular recognition were employed to bind liposomes to the functionalized quartz crystal. The QCM was used to distinguish between adsorption of vesicles and rupture due to strong adhesive forces. Intact vesicles display viscoelastic behaviour, while planar lipid bilayers as a result of vesicle rupture can be modelled by a thin rigid film. Furthermore, the adhesion of cells was modelled successfully by receptor bearing liposomes. Scanning force microscopy was used to confirm the results obtained by QCM measurements.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SHEAR MODE RESONATORS; HUMAN ANNEXIN-V; CELL-ADHESION; VESICLE AGGREGATION; MEMBRANE AGGREGATION; MOLECULAR-STRUCTURE; IMPEDANCE ANALYSIS; SURFACE-CHEMISTRY; MAMMALIAN-CELLS; WAVE RESONATORS; TSM resonator; scanning force microscopy; annexin; biotin-streptavidin; DODAB |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Jul 2021 12:17 |
| Last Modified: | 27 Jul 2021 12:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38481 |
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