Salamon, Daniel and Takacs, Maria and Schwarzmann, Fritz and Wolf, Hans and Minarovits, Janos and Niller, Hans Helmut (2003) High-resolution methylation analysis and in vivo Protein-DNA binding at the promoter of the viral oncogene LMP2A in B cell lines carrying latent Epstein-Barr virus genomes. VIRUS GENES, 27 (1). pp. 57-66. ISSN 0920-8569
Full text not available from this repository. (Request a copy)Abstract
Latency protein LMP2A of Epstein-Barr virus (EBV) has been implicated in EBV related tumorigenesis. To understand the host cell dependent expression of the LMP2A gene, it is necessary to analyse the regulatory mechanisms of the LMP2A promoter (LMP2Ap). By transient transfection and in vitro binding analyses two CBF1 sites have previously been shown to be involved in the regulation of LMP2Ap. However, the promoter structure has not been examined at the nucleotide level in vivo. Therefore we undertook a comprehensive analysis of in vivo protein binding and of CpG-methylation patterns at LMP2Ap in a panel of B cell lines carrying latent EBV genomes. The presence of characteristic footprints on two CBF1 and further binding-sites, together with overall hypomethylation of CpG dinucleotides correlated well with promoter activity. In contrast, the absence of several genomic footprints, as well as the presence of patches of highly methylated CpG dinucleotides were characteristic of silent LMP2Aps.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LIGATION-MEDIATED PCR; MEMBRANE-PROTEIN; NUCLEAR ANTIGEN-2; IMMORTALIZED LYMPHOCYTES; CPG METHYLATION; TRANSGENIC MICE; C PROMOTER; GENE; EXPRESSION; TRANSFORMATION; CpG methylation; Epstein-Barr virus; genomic footprints; viral latency |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Sep 2021 08:26 |
| Last Modified: | 06 Sep 2021 08:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38805 |
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