Hehlgans, Thomas and Muelller, Peter and Stopfer, Peter and Maennel, Daniela N. (2003) Activation of the lymphotoxin-beta receptor induces NF kappa B-dependent interleukin-6 and MIP-2 secretion in mouse fibrosarcoma cells. EUROPEAN CYTOKINE NETWORK, 14 (2). pp. 103-107. ISSN 1148-5493
Full text not available from this repository.Abstract
Activation of the lymphotoxin beta-receptor (LTbetaR), a member of the tumor necrosis factor receptor family, plays a crucial role in lymphoid organogenesis and tumor development. Lymphotoxin alpha(1)beta(2) (LTalpha(1)beta(2)) and LIGHT have been identified as membrane anchored ligands for the LTbetaR. While LTbetaR is expressed on a wide range of cell types e.g. fibroblasts and monocytes, the ligands are expressed only on activated lymphocytes and NK cells. In order to characterize LTbetaR expression and the biological consequences of LTbetaR activation rat anti-mouse LTbetaR monoclonal antibodies were generated. These antibodies recognized a mouse LTbetaR-Ig fusion protein as well as endogenous LTbetaR on a variety of mouse fibroblast and fibrosarcoma cell lines. Specificity was demonstrated by the lack of binding to LTbetaR-deficient embryonic fibrobasts. Competitive binding studies revealed that three different epitopes were recognized by the monoclonal antibodies. Two of the monoclonals activated the LTbetaR and induced activation of NFkappaB and secretion of MIP-2 and IL-6 in L929 mouse fibroblast cells. MIP-2 and IL-6 secretion was NFkappaB-dependent because IkappaB-transfected cells released significantly reduced amounts of both mediators.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TUMOR-NECROSIS-FACTOR; A375 MELANOMA-CELLS; T-CELL; 33-KDA GLYCOPROTEIN; HETEROMERIC COMPLEX; LYMPHOID-TISSUES; GROWTH ARREST; EXPRESSION; SURFACE; HYBRIDOMA; NF kappa B; MIP-2; IL-6 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Sep 2021 06:36 |
| Last Modified: | 15 Sep 2021 06:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39140 |
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