Tallen, G. and Riabowol, K. and Wolff, J. E. A. (2003) Expression of p33(ING1) mRNA and chemosensitivity in brain tumor cells. ANTICANCER RESEARCH, 23 (2B). pp. 1631-1635. ISSN 0250-7005
Full text not available from this repository. (Request a copy)Abstract
Background: Mutations and down-regulation of tumor suppressor genes can contribute to both tumorigensis and chemotherapy resistance. The tumor suppressor p33(ING1) has growth-inhibitory and pro-apoptotic effects recruiting p53 and it plays a role in DNA repair through interaction with PCNA. We questioned whether p33(ING1) mRNA expression correlates with the chemosensitivity of brain tumor cells. Materials and Methods: Various malignant brain tumor cell lines were examined for their sensitivity to cisplatin, doxorubicin, etoposide and the antimitotic agents vincristine and paclitaxe by MTT-cytotoxicity assays. p33(ING1) mRAA expression was determined by RT-PCR. Results: We found that, unlike other tumor types, ING1 levels were higher in glioma cell lines than in normal control cells. Medulloblastoma cells revealed the lowest ING1 expression of the lines tested. Comparing all cell lines, p33(ING1) gene expression significantly (p=0.028) correlated with resistance to vincristine (r(2) = 0.87). Conclusion: Our results suggest that p33(ING1) mRNA levels may be used to predict the chemosensitivity of brain tumor cells to vincristine.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MUTANT P53 PROTEIN; BREAST-CANCER; ING1; SUPPRESSOR; APOPTOSIS; GROWTH; LINE; IDENTIFICATION; CARCINOMAS; GLIOMAS; ING1; tumor suppressor genes; brain tumor; chemosensitivity |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kinder- und Jugendmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Sep 2021 10:34 |
| Last Modified: | 15 Sep 2021 10:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39216 |
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