Sheppard, Carol and Blombach, Fabian and Belsom, Adam and Schulz, Sarah and Daviter, Tina and Smollett, Katherine and Mahieu, Emilie and Erdmann, Susanne and Tinnefeld, Philip and Garrett, Roger and Grohmann, Dina and Rappsilber, Juri and Werner, Finn (2016) Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP. NATURE COMMUNICATIONS, 7: 13595. ISSN 2041-1723,
Full text not available from this repository. (Request a copy)Abstract
Little is known about how archaeal viruses perturb the transcription machinery of their hosts. Here we provide the first example of an archaeo-viral transcription factor that directly targets the host RNA polymerase (RNAP) and efficiently represses its activity. ORF145 from the temperate Acidianus two-tailed virus (ATV) forms a high-affinity complex with RNAP by binding inside the DNA-binding channel where it locks the flexible RNAP clamp in one position. This counteracts the formation of transcription pre-initiation complexes in vitro and represses abortive and productive transcription initiation, as well as elongation. Both host and viral promoters are subjected to ORF145 repression. Thus, ORF145 has the properties of a global transcription repressor and its overexpression is toxic for Sulfolobus. On the basis of its properties, we have re-named ORF145 RNAP Inhibitory Protein (RIP).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN SECONDARY STRUCTURE; TRANSCRIPTIONAL REGULATOR; CIRCULAR-DICHROISM; MASS-SPECTROMETRY; CROSS-LINKING; T7 GP2; VIRUSES; MECHANISM; COMPLEX; PROCESSIVITY; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Dina Grohmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Apr 2019 10:04 |
| Last Modified: | 12 Apr 2019 10:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3934 |
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