Nickell, Stephan and Hegerl, Reiner and Baumeister, Wolfgang and Rachel, Reinhard (2003) Pyrodictium cannulae enter the periplasmic space but do not enter the cytoplasm, as revealed by cryo-electron tomography. JOURNAL OF STRUCTURAL BIOLOGY, 141 (1): PII S1047-. pp. 34-42. ISSN 1047-8477
Full text not available from this repository. (Request a copy)Abstract
The hyperthermophilic archaeon Pyrodictium grows in the form of a macroscopically visible network. It consists of cells entrapped in an extracellular matrix of hollow tubules, the "cannulae." Here, we present the three-dimensional structure of a single cell in conjunction with two extracellular cannulae, as determined by cryo-electron microscopy. To achieve this, the information from two independent tilt series of the same specimen was combined, with the specimen rotated in the second series. In the three-dimensional tomographic reconstruction, we were able to trace the two carmulae in their full length, in particular, also inside the cell. One cannula enters the periplasmic space, while the other cannula contacts the surface of the cell, the S-layer. This indicates that the cannulae interconnect individual cells with each other on the level of their periplasmic space; we do not, however, have evidence that they enter the cytoplasm of the cells. The implications of these data for possible functions of the carmulae are discussed. (C) 2002 Elsevier Science (USA). All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN; MICROSCOPY; ICE; TRANSPORTERS; VESICLES; ABYSSI; LAYER; 105-DEGREES-C; BIOSYNTHESIS; GLYCOPROTEIN; archaea; cannulae; cryo-electron tomography; double tilt; periplasmic space; Pyrodictium; 3D reconstruction |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Sep 2021 13:20 |
| Last Modified: | 22 Sep 2021 13:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39485 |
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