Stoehr, Robert and Wild, Peter and Hartmann, Arndt (2003) Lasermicrodissection - An important prerequisite for the molecular-genetic analysis of bladder cancer. PATHOLOGY RESEARCH AND PRACTICE, 199 (6). pp. 355-362. ISSN 0344-0338
Full text not available from this repository. (Request a copy)Abstract
The past 10 years have shown that a crucial point in the molecular-genetic analysis of malignant tumors is the exact histopathological definition and separation of the investigated lesions. Laser microdissection has become an important tool allowing for the study of specific histological entities and defined preneoplastic lesions. Reliable detection of tumor-specific alterations can be compromised by the presence of normal cells. This requires microdissection of pure tumor cell populations (>80%), necessary for detecting chromosomal alterations by loss of heterozygosity analysis (LOH) and fluorescence in situ hybridization (FISH). The combination of microdissection and molecular methods could also be useful for studying multifocal lesions of the urothelial tract. This article describes in detail the use of laser microdissection, whole genome amplification by Improved Primer Extension Preamplification (I-PEP)-PCR and subsequent LOH, FISH, and sequencing analyses for the investigation of urothelial tumors and their precursors, e.g. dysplasias and hyperplasias. The combination of the described methods allows for a wide spectrum of molecular investigations in lesions with a limited number of tumor cells, and might help to understand the fundamental alterations involved in urothelial carcinogenesis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MULTIFOCAL UROTHELIAL CARCINOMAS; HUMAN URINARY-BLADDER; IN-SITU; TUMOR PROGRESSION; DELETION ANALYSIS; FGFR3 MUTATION; CELL-CARCINOMA; DYSPLASIA; SPREAD; MICROSATELLITE; lasermicrodissection; bladder cancer; clonality; LOH analysis; prenoplastic lesions |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Sep 2021 09:19 |
| Last Modified: | 28 Sep 2021 09:19 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39514 |
Actions (login required)
 |
View Item |
