Sester, Martina and Sester, Urban and Koehler, Hans and Schneider, Thomas and Deml, Ludwig and Wagner, Ralf and Mueller-Lantzsch, Nikolaus and Pees, Hans W. and Meyerhans, Andreas (2000) Rapid whole blood analysis of virus-specific CD4 and CD8 T cell responses in persistent HIV infection. AIDS, 14 (17). pp. 2653-2660. ISSN 0269-9370
Full text not available from this repository.Abstract
Objectives: Upon HIV infection, strong antiviral cytotoxic and helper T cell responses are generated. They are considered to be an important component in the control of HIV viral load. A simple and rapid whole blood assay was established to quantify and simultaneously characterize HIV-reactive CD4 and CD8 cells. The assay was applied to evaluate the effect of antiretroviral therapy on HIV-specific T cell responses. Methods: Whole blood of 33 HIV-infected individuals was specifically stimulated by HIV-1 pr55(gag), and activation-induced intracellular cytokine expression in CD4 and CD8 T cells was analysed by flow cytometry. Results: HIV-l-specific CD8 and CD4 T cells can be quantified simultaneously. As specific antigen, HIV-1 Pr55(gag) virus-like particles were superior to soluble protein, especially for the activation of CD8 T cells. In untreated individuals, a high frequency of HIV-specific T cells was observed. The frequency of CD8 T cells was consistently higher than the respective CD4 T cell response, thus demonstrating a dominance in CD8 T cell expansion in persistent HIV infection. Patients on antiretroviral therapy showed a significant reduction in HIV-specific CD4 and, even more strikingly, CD8 T cells. Conclusion: The whole blood assay provides a rapid estimate of the total antiviral T cell resources, and is highly suited for a clinical setting. It may thus have widespread applications for the evaluation of vaccination strategies and immunotherapy. Because antiretroviral therapy significantly reduces both HIV-specific cytotoxic and helper T cell responses, future therapeutic strategies should aim at improving cellular antiviral immunity. (C) 2000 Lippincott Williams & Wilkins.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CLASS-I; ANTIRETROVIRAL THERAPY; LYMPHOCYTE ACTIVITY; PEPTIDE COMPLEXES; TYPE-1 INFECTION; IMMUNE-RESPONSES; DENDRITIC CELLS; FLOW-CYTOMETRY; IMMUNODEFICIENCY; ANTIGEN; antiretroviral therapy; CD4; CD8; cellular immunity; HIV |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Mar 2022 17:02 |
| Last Modified: | 09 Mar 2022 17:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41983 |
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