Enderlein, Joerg (2000) Theoretical study of detection of a dipole emitter through an objective with high numerical aperture. OPTICS LETTERS, 25 (9). pp. 634-636. ISSN 0146-9592
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The problem of detecting electric dipole emitters (such as a fluorescing molecule) through a microscope objective with a high numerical aperture is considered. Exact vector-wave-optics results of the field distribution of the electromagnetic field in image space are derived. On the basis of these results, the collection-efficiency function (CEF) is calculated. This calculation is compared with a semigeometric approximation of the CEF, for which a new analytical formula is presented. The derived results are important for applications such as fluorescence correlation spectroscopy and single-molecule fluorescence detection with a confocal microscope. (C) 2000 Optical Society of America.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SINGLE MOLECULES; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 May 2022 10:02 |
| Last Modified: | 10 May 2022 10:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42544 |
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