Eckl, Daniel B. and Huber, Harald and Baeumler, Wolfgang (2020) First Report on Photodynamic Inactivation of Archaea Including a Novel Method for High-Throughput Reduction Measurement. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 96 (4). pp. 883-889. ISSN 0031-8655, 1751-1097
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Archaea are considered third, independent domain of living organisms besides eukaryotic and bacterial cells. To date, no report is available of photodynamic inactivation (PDI) of any archaeal cells. Two commercially available photosensitizers (SAPYR and TMPyP) were used to investigate photodynamic inactivation of Halobacterium salinarum. In addition, a novel high-throughput method was tested to evaluate microbial reduction in vitro. Due to the high salt content of the culture medium, the physical and chemical properties of photosensitizers were analyzed via spectroscopy and fluorescence-based DPBF assays. Attachment or uptake of photosensitizers to or in archaeal cells was investigated. The photodynamic inactivation of Halobacterium salinarum was evaluated via growth curve method allowing a high throughput of samples. The presented results indicate that the photodynamic mechanisms are working even in high salt environments. Either photosensitizer inactivated the archaeal cells with a reduction of 99.9% at least. The growth curves provided a fast and precise measurement of cell viability. The results show for the first time that PDI can kill not only bacterial cells but also robust archaea. The novel method for generating high-throughput growth curves provides benefits for future research regarding antimicrobial substances in general.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | METHANOGENIC BACTERIA; CANDIDA-ALBICANS; SINGLET OXYGEN; LIPIDS; PHOTOSENSITIZATION; PHOTOINACTIVATION; SUSCEPTIBILITY; MECHANISMS; RHODOPSIN; MEMBRANE; |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Mar 2021 10:09 |
| Last Modified: | 26 Mar 2021 10:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44744 |
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