Wert, Stefan and Iken, Heiko and Schoening, Michael J. and Matysik, Frank-Michael (2021) Development of a Temperature-pulse Enhanced Electrochemical Glucose Biosensor and Characterization of its Stability via Scanning Electrochemical Microscopy. ELECTROANALYSIS, 33 (5). pp. 1143-1151. ISSN 1040-0397, 1521-4109
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Glucose oxidase (GOx) is an enzyme frequently used in glucose biosensors. As increased temperatures can enhance the performance of electrochemical sensors, we investigated the impact of temperature pulses on GOx that was drop-coated on flattened Pt microwires. The wires were heated by an alternating current. The sensitivity towards glucose and the temperature stability of GOx was investigated by amperometry. An up to 22-fold increase of sensitivity was observed. Spatially resolved enzyme activity changes were investigated via scanning electrochemical microscopy. The application of short (<100 ms) heat pulses was associated with less thermal inactivation of the immobilized GOx than long-term heating.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REDOX COMPETITION; ELECTRODES; OXIDASE; KINETICS; VOLTAMMETRY; DEPOSITION; SILICA; CELLS; DNA; Biosensors; Electrochemistry; Thermoelectrochemistry; Scanning electrochemical microscopy |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Aug 2022 11:22 |
| Last Modified: | 17 Aug 2022 11:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46618 |
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