Lehnfeld, J. and Dukashin, Y. and Mark, J. and White, G. D. and Wu, S. and Katzur, V and Mueller, Rainer and Ruhl, S. (2021) Saliva and Serum Protein Adsorption on Chemically Modified Silica Surfaces. JOURNAL OF DENTAL RESEARCH, 100 (10): 0022034521. pp. 1047-1054. ISSN 0022-0345, 1544-0591
Full text not available from this repository. (Request a copy)Abstract
Biomaterials, once inserted in the oral cavity, become immediately covered by a layer of adsorbed proteins that consists mostly of salivary proteins but also of plasma proteins if the biomaterial is placed close to the gingival margin or if it becomes implanted into tissue and bone. It is often this protein layer, rather than the pristine biomaterial surface, that is subsequently encountered by colonizing bacteria or attaching tissue cells. Thus, to study this important initial protein adsorption from human saliva and serum and how it might be influenced through chemical modification of the biomaterial surface, we have measured the amount of protein adsorbed and analyzed the composition of the adsorbed protein layer using gel electrophoresis and western blotting. Here, we have developed an in vitro model system based on silica surfaces, chemically modified with 7 silane-based self-assembled monolayers that span a broad range of physicochemical properties, from hydrophilic to hydrophobic surfaces (water contact angles from 15 degrees to 115 degrees), low to high surface free energy (12 to 57 mN/m), and negative to positive surface charge (zeta potentials from -120 to +40 mV at physiologic pH). We found that the chemical surface functionalities exerted a substantial effect on the total amounts of proteins adsorbed; however, no linear correlation of the adsorbed amounts with the physicochemical surface parameters was observed. Only the adsorption behavior of a few singular protein components, from which physicochemical data are available, seems to follow physicochemical expectations. Examples are albumin in serum and lysozyme in saliva; in both, adsorption was favored on countercharged surfaces. We conclude from these findings that in complex biofluids such as saliva and serum, adsorption behavior is dominated by the overall protein-binding capacity of the surface rather than by specific physicochemical interactions of single protein entities with the surface.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SELF-ASSEMBLED MONOLAYERS; HYDROPHOBICITY; PELLICLES; COATINGS; BACTERIA; BRUSHES; IMPACT; ENERGY; biomaterials; surface properties; self-assembled monolayers; pellicle; protein amount; protein composition |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > PD Dr. Rainer Müller |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Sep 2022 08:55 |
| Last Modified: | 09 Sep 2022 08:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46902 |
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