Zhang, Jun and Geiger, Yasemin and Sotier, Florian and Djordjevic, Sasa and Docheva, Denitsa and Sudhop, Stefanie and Clausen-Schaumann, Hauke and Huber, Heinz P. (2021) Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations. MICROMACHINES, 12 (10): 1172. ISSN , 2072-666X
Full text not available from this repository. (Request a copy)Abstract
Femtosecond laser pulses have been successfully used for film-free single-cell bioprinting, enabling precise and efficient selection and positioning of individual mammalian cells from a complex cell mixture (based on morphology or fluorescence) onto a 2D target substrate or a 3D pre-processed scaffold. In order to evaluate the effects of higher pulse durations on the bioprinting process, we investigated cavitation bubble and jet dynamics in the femto- and picosecond regime. By increasing the laser pulse duration from 600 fs to 14.1 ps, less energy is deposited in the hydrogel for the cavitation bubble expansion, resulting in less kinetic energy for the jet propagation with a slower jet velocity. Under appropriate conditions, single cells can be reliably transferred with a cell survival rate after transfer above 95% through the entire pulse duration range. More cost efficient and compact laser sources with pulse durations in the picosecond range could be used for film-free bioprinting and single-cell transfer.</p>
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INDUCED BREAKDOWN THRESHOLDS; FILM-FREE LASER; CAVITATION BUBBLES; 1ST-ORDER MODEL; AQUEOUS-MEDIA; WATER; COMPUTATION; NANOSECOND; MECHANISMS; LIQUIDS; laser-induced forward transfer (LIFT); film-free LIFT; single-cell bioprinting; tissue engineering; femtosecond laser-based bioprinting; picosecond laser-based bioprinting |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Sep 2022 06:33 |
| Last Modified: | 21 Sep 2022 06:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47770 |
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