Hengerer, Arne and Decker, Jochen and Prohaska, Elke and Hauck, Sabine and Koesslinger, Conrad and Wolf, Hans (1999) Quartz crystal microbalance (QCM) as a device for the screening of phage libraries. BIOSENSORS & BIOELECTRONICS, 14 (2). pp. 139-144. ISSN 0956-5663,
Full text not available from this repository. (Request a copy)Abstract
An immunosensing system based on a quartz crystal microbalance (QCM) is presented for the selection of both antigen specific recombinant antibodies and antigen specific human pancreatic secretory trypsin inhibitor (hPSTI) mutants isolated from large phage libraries. The QCM was integrated into a flow injection analysis system for the straightforward analysis of large sample numbers. Measurements were performed using a biotinylated antigen immobilized by streptavidin onto the gold surface of the quartz crystal and phages displaying recombinant antibodies or hPSTI mutants. The results obtained by the QCM were in accordance to those of a well established enzyme linked immunosorbent assay (ELISA). Therefore, the QCM is well suited for the detection of single high affinity clones isolated from large phage display libraries. (C) 1999 Elsevier Science S.A. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ANTIBODIES; BIOSENSOR; immunosensors; quartz crystal microbalance; recombinant antibodies; phage display; single chain antibodies; human pancreatic secretory trypsin inhibitor |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Nov 2022 07:47 |
| Last Modified: | 22 Nov 2022 07:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48536 |
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