Marschall, Manfred and Helten, Anke and Hechtfischer, Anne and Zach, Anke and Banaschewski, Christine and Hell, Wolfgang and Meier-Ewert, Herbert (1999) The ORF, regulated synthesis, and persistence-specific variation of influenza C viral NS1 protein. VIROLOGY, 253 (2). pp. 208-218. ISSN 0042-6822,
Full text not available from this repository. (Request a copy)Abstract
The open reading frame (ORF) and the regulated synthesis of the influenza C viral NS1 protein were analyzed in view of viruses possessing different biological activities. We provide evidence for a 246-amino-acid NS1-ORF, encoded by five viral strains and variants. Prokaryotic expression of the prototype NS1-ORF resulted in a product of 27 kDa, confirming the predicted molecular weight. Using an antiserum raised against recombinant NS1 protein, nonstructural proteins of wild-type virus were detected in infected cells for a limited course of time, whereas a persistent virus variant was characterized by a long-term nonstructural gene expression. As examined by infection experiments, the intracellular distribution of nonstructural protein was nuclear and cytoplasmic, whereas in NS1 gene-transfected cells, the cytoplasmic localization occurred in a fine-grained structure, suggesting an analogy to influenza A viral NS1 protein. Concerning persistent infection, NS1 protein species differing in sizes and posttranslational modifications were observed for a persistent virus variant, as particularly illustrated by a high degree of NS1 phosphorylation. Virus reassortant analyses proved the importance of the NS-coding genomic segment: the minimal viral properties required for the establishment of persistence were transferred with this segment to a monoreassortant virus. Thus the influenza C viral NS1 protein is a 246-amino-acid nuclear-cytoplasmic phosphoprotein that can be subject to specific variations being functionally linked to a persistent virus phenotype. (C) 1999 Academic Press.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESSENGER-RNA; NUCLEOCYTOPLASMIC TRANSPORT; NONSTRUCTURAL PROTEIN; KIDNEY-CELLS; A VIRUS; GENE; INFECTION; TRANSLATION; REPLICATION; LEVEL; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Nov 2022 14:57 |
| Last Modified: | 22 Nov 2022 14:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48579 |
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