Gerhard, Markus and Lehn, Norbert and Neumayer, Nina and Boren, Thomas and Rad, Roland and Schepp, Wolfgang and Miehlke, Stephan and Classen, Meinhard and Prinz, Christian (1999) Clinical relevance of the Helicobacter pylori gene for blood-group antigen-binding adhesin. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 96 (22). pp. 12778-12783. ISSN 0027-8424,
Full text not available from this repository. (Request a copy)Abstract
Infection with Helicobacter pylori is associated with different human gastric diseases. Biochemical studies, in vitro adherence assays, and in vivo animal models revealed that epithelial attachment of H. pylori can be mediated by the blood-group antigen-binding adhesin (BabA) targeting human Lewis(b) surface epitopes, Studies with transgenic mice expressing the Lewis(b) epitope have shown that such attachment can alter disease outcome. In the current study, the presence of the babA2 gene encoding the adhesin was investigated in clinical isolates from a German population by using PCR and reverse transcription-PCR. A positive genotype was correlated to allelic variations in the genes encoding VacA and CagA and also to the prevalence of duodenal ulcer, distal gastric: adenocarcinoma, mucosa-associated lymphoid tissue lymphoma, and antral gastritis. The presence of babA2 was significantly associated with duodenal ulcer (P = 0.0002) and adenocarcinoma (P = 0.033). In contrast, type 1 strains (vacAs1- and cagA-positive) were associated with only duodenal ulcer (P = 0.004) but not adenocarcinoma (P = 0.235). Genotype presence of babA2, vacAs1, and cagA ("triple-positive" strains) showed a highly significant correlation to the prevalence of ulcer (P = 0.000002) and adenocarcinoma (P = 0.014) and discriminated significantly better between disease outcome than did the current type 1 classification. These results indicate that the babA2 gene is of high clinical relevance and would he a useful marker to identify patients who are at higher risk for specific H. pylori-related diseases.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LYMPHOID-TISSUE LYMPHOMA; HUMAN GASTRIC EPITHELIUM; VACUOLATING CYTOTOXIN; INTESTINAL METAPLASIA; PEPTIC-ULCERATION; VIRULENCE FACTORS; CELL LINEAGE; CAGA; EXPRESSION; INFECTION; VacA; CagA; ulcer; adenocarcinoma |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Dec 2022 13:09 |
| Last Modified: | 13 Dec 2022 13:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48913 |
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