Menet, Anne and Speth, Cornelia and Larcher, Clara and Prodinger, Wolfgang M. and Schwendinger, Michael G. and Chan, Philippe and Jaeger, Michael and Schwarzmann, Fritz and Recheis, Heidrun and Fontaine, Marc and Dierich, Manfred P. (1999) Epstein-Barr virus infection of human astrocyte cell lines. JOURNAL OF VIROLOGY, 73 (9). pp. 7722-7733. ISSN 0022-538X, 1098-5514
Full text not available from this repository. (Request a copy)Abstract
Epstein-Barr virus (EBV) is implicated in different central nervous system syndromes. The major cellular receptor for EBV, complement receptor type 2 (CR2) (CD21), is expressed by different astrocyte cell lines and human fetal astrocytes, suggesting their susceptibility to EBV infection. We demonstrated the infection of two astrocyte cell lines, T98 and CB193, at low levels. As infection was mediated by CR2, we used two stable CR2 transfectant astrocyte cell lines (T98CR2 and CB193CR2) to achieve a more efficient infection. We have monitored EBV gene expression for 2 months and observed the transient infection of T98 and T98CR2 cells and persistent infection of CB193 and CB193CR2 cells. The detection of BZLF1, BALF2, and BcLF1 mRNA expression suggests that the lytic cycle is initiated at early time points postinfection. At later time points the pattern of mRNA expressed (EBER1, EBNA1, EBNA2, and LMP1) differs from latency type III in the absence of LMP2A transcription and in the expression of BALF2 and BcLF1 but not BZLF1. A reactivation of the lytic cycle was achieved in CB193CR2 cells by the addition of phorbol esters. These studies identify astrocyte cell lines as targets for EBV infection and suggest that this infection might play a role in the pathology of EBV in the brain.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN-IMMUNODEFICIENCY-VIRUS; POLYMERASE CHAIN-REACTION; CENTRAL-NERVOUS-SYSTEM; AIDS-RELATED LYMPHOMAS; HUMAN LYMPHOCYTES-B; SWITCH GENE BZLF1; NASOPHARYNGEAL CARCINOMA; NEGATIVE REGULATION; LYTIC REPLICATION; RECEPTOR CR-2; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Jan 2023 05:43 |
| Last Modified: | 11 Jan 2023 05:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49043 |
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