Paulus, Christina and Hellebrand, Susanne and Tessmer, Uwe and Wolf, Hans and Kraeusslich, Hans-Georg and Wagner, Ralf (1999) Competitive inhibition of human immunodeficiency virus type-1 protease by the Gag-Pol transframe protein. JOURNAL OF BIOLOGICAL CHEMISTRY, 274 (31). pp. 21539-21543. ISSN 0021-9258,
Full text not available from this repository. (Request a copy)Abstract
The human immunodeficiency virus type-1 (HIV-1) transframe protein p6* is located between the structural and enzymatic domains of the Gag-Pol polyprotein, Banked by the nucleocapsid (NC) and the protease (PR) domain at its amino and carboxyl termini, respectively. Here, we report that recombinant highly purified HIV-1 p6* specifically inhibits mature HIV-1 PR activity. Kinetic analyses and cross-linking experiments revealed a competitive mechanism for PR inhibition by p6*. We further demonstrate that the four carboxyl-terminal residues of p6* are essential but not sufficient for p6*-mediated inhibition of PR activity. Based on these results, we suggest a role of the transframe protein p6* in regulating HIV-1 PR activity during viral replication.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HIV-1 PROTEASE; VIRAL INFECTIVITY; RETROVIRAL PROTEASES; PARTICLE FORMATION; ASPARTIC PROTEASE; POLYPROTEIN; EXPRESSION; SEQUENCE; PRECURSOR; DIMER; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Jan 2023 13:51 |
| Last Modified: | 24 Jan 2023 13:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49116 |
Actions (login required)
 |
View Item |
