Weinberger, Klaus M. and Zoulek, Gert and Bauer, Tanja and Böhm, Stephan and Jilg, Wolfgang (1999) A novel deletion mutant of hepatitis B virus surface antigen. JOURNAL OF MEDICAL VIROLOGY, 58 (2). pp. 105-110. ISSN 0146-6615,
Full text not available from this repository. (Request a copy)Abstract
HBsAg is the most important serological marker for acute or chronic hepatitis B. Nevertheless, there are reports of HBsAg-negative virus carriers, either with anti-HBc as the only marker for hepatitis B virus (HBV) infection or even positive for anti-HBs and anti-HBc. We report isolates from a patient, in which a deletion in the HBs-gene was associated with persisting viremia in the presence of anti-HBs. The 62-year-old female, infected most likely by her husband, had detectable markers of chronic active hepatitis B, such as HBsAg, HBeAg, and anti-HBc-IgM, for 2 years. The patient then seroconverted to anti-HBs, although HBeAg and anti-HBc-IgM remained detectable. At this time, semiquantitative polymerase chain reaction showed about 10(4) viral genomes per milliliter of serum. Direct sequencing of the amplified products revealed a major population of DNA molecules with a deletion of nucleotide 31 of the HBs-gene, which up to now has not been described. This deletion led to a frame-shift and introduced a stop-codon after 21 amino acids of the sHBsAg. We suspect that this deletion, and the resulting HBsAg lacking the major epitopes recognized by specific antibodies, could favor ongoing viral replication, despite the presence of anti-HBs. However, because the reading frame of the polymerase was also severely damaged by this deletion, it is assumed that a minor population of intact genomes was present to help in the formation of virus particles. J. Med. Virol. 58:105-110, 1999. (C) 1999 Wiley-Liss, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHRONIC LIVER-DISEASE; INDUCED ESCAPE MUTANT; CORE ANTIGEN; HBS-ANTIGEN; ANTIBODIES; INFECTION; BLOOD; SERA; DNA; PCR; serology; genetic variant; deletion mutant; direct sequencing; clonal analysis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Feb 2023 09:59 |
| Last Modified: | 14 Feb 2023 09:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49209 |
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