Schmidt, Martin and Löffler, Georg (1998) Induction of aromatase activity in human adipose tissue stromal cells by extracellular nucleotides - Evidence for P2-purinoceptors in adipose tissue. EUROPEAN JOURNAL OF BIOCHEMISTRY, 252 (1). pp. 147-154. ISSN 0014-2956
Full text not available from this repository.Abstract
Here we describe properties of the P2-purinoceptor, which is involved in the regulation of the key enzyme of estrogen biosynthesis, aromatase cytochrome P-450, in stromal cells from human adipose tissue. Aromatase activity induced by cortisol and platelet-derived growth factor BB (PDGF-BB) is further increased by addition of ATP, ADP, AMP and, albeit with reduced potency, adenosine,, GTP and adenosine(5')tetraphospho(5') adenosine. Stable P1-purinoceptor agonists are inactive, whereas P2X-purinoceptor agonists mimic the effects of purine(s) (nucleotides). Priorincubation of cells with a P2-purinoceptor antagonist, but not P1-purinoceptor antagonists, blocks augmentation of aromatase activity by all ligands. Nucleotides, but not adenosine, retain their ability to augment aromatase activity in the presence of adenosine deaminase, indicating that they do not act via their metabolite adenosine. These results lead to the conclusion that at least one member of the P2-subclass of purinoceptors exists in adipose tissue and is involved in modulation of aromatase activity in vitro. The pharmacological profile of the P2-site differs from these reported for cloned P2-purinoceptors, but is similar to that of the P2X-subclass. Therefore, a combined response of different members of the P2X-purinoceptor subclass or of members of different P2-purinoceptor subclasses cannot be discounted. Purinoceptor activation triggers cytoplasmic calcium transients, which, in contrast to aromatase induction, are independent from the presence of cortisol and PDGF-BB. Therefore the involved P2-purinoceptor(s) seem(s) to be constitutively expressed in human adipose tissue stromal cells. P2-purinoceptor(s) might provide a direct link between sympathetic nerve activity and estrogen biosynthesis in human adipose tissue. Furthermore it (or they) may contribute to the regulation of lipolysis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GATED ION CHANNELS; ATP RECEPTOR; ESTROGEN BIOSYNTHESIS; ADENOSINE; EXPRESSION; ANDROSTENEDIONE; CONVERSION; PROTEIN; CLONING; CYTOCHROME-P450; aromatase cytochrome P-450; purinoceptor; nucleotide signaling; calcium mobilization; adipose tissue (human) |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Feb 2023 09:01 |
| Last Modified: | 28 Feb 2023 09:01 |
| URI: | https://pred.uni-regensburg.de/id/eprint/50081 |
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