LUPPA, P and JACOB, K and EHRET, W (1993) THE PRODUCTION OF PORPHYRINS FROM DELTA-AMINOLEVULINIC-ACID BY HAEMOPHILUS-PARAINFLUENZAE. JOURNAL OF MEDICAL MICROBIOLOGY, 39 (4). pp. 262-267. ISSN 0022-2615,
Full text not available from this repository.Abstract
Porphyrin production by the haemin-independent Haemophilus parainfluenzae in the diagnostic porphyrin test, which determines the X-factor requirement in Haemophilus spp., was analysed quantitatively by applying modem high-performance liquid chromatographic (HPLC) methods. Ion-pair reversed-phase HPLC enabled the simultaneous separation of all porphyrin intermediates and their isomers of haem biosynthesis produced by the bacteria. The pH-dependence of porphyrin production and the respective composition of the porphyrins within the bacterial cells and in the supernate were investigated. A pH optimum of 6.9-8.0 for the production of porphyrins was found and there were marked differences in the porphyrin profiles at different pH values.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PERFORMANCE LIQUID-CHROMATOGRAPHY; ISOMERS; IV; PH; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53748 |
Actions (login required)
 |
View Item |
