SCHMALZ, G and SCHWEIKL, H (1990) A SEMIAUTOMATED CELL-CULTURE EVALUATION SYSTEM FOR CYTOTOXICITY TESTING OF DENTAL MATERIALS. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 1 (4). pp. 228-232. ISSN 0957-4530,
Full text not available from this repository.Abstract
A rapid and quantitative determination of viable cells in the cytotoxicity testing of dental materials is desirable to evaluate large numbers of samples in a short time. For this purpose a new and semi-automated cell culture evaluation system was developed using the fluorogenic dye fluorescein diacetate (FDA) for vital staining and microtiter plates for culture vessels. Our methodological experiments showed that in this system the fluorescence intensity was linearily related to the cell number from 500 to 20 000 cells per culture-1 and that fluorescence recording was stable for between at least 1 to 2h using a quencher solution for absorbing extracellular fluorescence. The results from toxicity testing of different dental materials (two glass ionomer cements, a phosphate cement, a composite resin and monomeric methylmethacrylate) corresponded to those derived from other, standard test methods. Because of the ease of performance, the quantitative, rapid evaluation system and the small culture vessels requiring only few cells per culture, the test method presented may be an interesting alternative to other cell culture techniques for cytotoxicity testing.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/55235 |
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