Weiss, Frederik and Schnabel, Anke and Planatscher, Hannes and van den Berg, Bart H. J. and Serschnitzki, Bettina and Nuessler, Andreas K. and Thasler, Wolfgang E. and Weiss, Thomas S. and Reuss, Matthias and Stoll, Dieter and Templin, Markus F. and Joos, Thomas O. and Marcus, Katrin and Poetz, Oliver (2015) Indirect protein quantification of drug-transforming enzymes using peptide group-specific immunoaffinity enrichment and mass spectrometry. SCIENTIFIC REPORTS, 5: 8759. ISSN 2045-2322,
Full text not available from this repository. (Request a copy)Abstract
Immunoaffinity enrichment of proteotypic peptides, coupled with selected reaction monitoring, enables indirect protein quantification. However the lack of suitable antibodies limits its widespread application. We developed a method in which multi-specific antibodies are used to enrich groups of peptides, thus facilitating multiplexed quantitative protein assays. We tested this strategy in a pharmacokinetic experiment by targeting a group of homologous drug transforming proteins in human hepatocytes. Our results indicate the generic applicability of this method to any biological system.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SIMULTANEOUS ABSOLUTE QUANTIFICATION; PERFORMANCE LIQUID-CHROMATOGRAPHY; HUMAN LIVER; HUMAN HEPATOCYTES; UDP-GLUCURONOSYLTRANSFERASES; CYTOCHROME-P450 ENZYMES; TARGETED PROTEOMICS; EXPRESSION; INDUCTION; TRANSPORTERS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kinder- und Jugendmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Jul 2019 07:47 |
| Last Modified: | 24 Jul 2019 07:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5806 |
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