Bauswein, Markus and Eidenschink, Lisa and Knoll, Gertrud and Neumann, Bernhard and Angstwurm, Klemens and Zoubaa, Saida and Riemenschneider, Markus J. and Lampl, Benedikt M. J. and Pregler, Matthias and Niller, Hans Helmut and Jantsch, Jonathan and Gessner, Andre and Eberhardt, Yvonne and Huppertz, Gunnar and Schramm, Torsten and Kuehn, Stefanie and Koller, Michael and Drasch, Thomas and Ehrl, Yvonne and Banas, Bernhard and Offner, Robert and Schmidt, Barbara and Banas, Miriam C. (2023) Human Infections with Borna Disease Virus 1 (BoDV-1) Primarily Lead to Severe Encephalitis: Further Evidence from the Seroepidemiological BoSOT Study in an Endemic Region in Southern Germany. VIRUSES-BASEL, 15 (1): 188. ISSN , 1999-4915
Full text not available from this repository. (Request a copy)Abstract
More than 40 human cases of severe encephalitis caused by Borna disease virus 1 (BoDV-1) have been reported to German health authorities. In an endemic region in southern Germany, we conducted the seroepidemiological BoSOT study ("BoDV-1 after solid-organ transplantation") to assess whether there are undetected oligo- or asymptomatic courses of infection. A total of 216 healthy blood donors and 280 outpatients after solid organ transplantation were screened by a recombinant BoDV-1 ELISA followed by an indirect immunofluorescence assay (iIFA) as confirmatory test. For comparison, 288 serum and 258 cerebrospinal fluid (CSF) samples with a request for tick-borne encephalitis (TBE) diagnostics were analyzed for BoDV-1 infections. ELISA screening reactivity rates ranged from 3.5% to 18.6% depending on the cohort and the used ELISA antigen, but only one sample of a patient from the cohort with requested TBE diagnostics was confirmed to be positive for anti-BoDV-1-IgG by iIFA. In addition, the corresponding CSF sample of this patient with a three-week history of severe neurological disease tested positive for BoDV-1 RNA. Due to the iIFA results, all other results were interpreted as false-reactive in the ELISA screening. By linear serological epitope mapping, cross-reactions with human and bacterial proteins were identified as possible underlying mechanism for the false-reactive ELISA screening results. In conclusion, no oligo- or asymptomatic infections were detected in the studied cohorts. Serological tests based on a single recombinant BoDV-1 antigen should be interpreted with caution, and an iIFA should always be performed in addition.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ELEMENTS; PROTEIN; Borna disease virus 1 (BoDV-1); epidemiology; encephalitis; diagnostics; ELISA; indirect immunofluorescence assay (iIFA); solid organ transplantation; linear epitope mapping; molecular mimicry; endogenous Borna-like elements |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Nephrologie Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Medicine > Lehrstuhl für Neurologie Medicine > Abteilung für Neuropathologie Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Klinische Studien Medicine > Institut für Epidemiologie und Präventivmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Mar 2024 10:26 |
| Last Modified: | 08 Mar 2024 10:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/59129 |
Actions (login required)
 |
View Item |
