Thaus, Christopher and Lubnow, Matthias and Krenkel, Lars and Lehle, Karla (2025) Material-Induced Platelet Adhesion/Activation and Hemolysis of Membrane Lung Components from Extracorporeal Membrane Oxygenation. BIOMEDICINES, 13 (10): 2323. ISSN , 2227-9059
Full text not available from this repository. (Request a copy)Abstract
Background: Contact between blood and the large artificial surfaces within membrane lungs (MLs) is one reason for device-induced thrombus formation during extracorporeal membrane oxygenation (ECMO). Methods: Hemocompatibility testing of gas-exchange fibers (GFs) and heat-exchange fibers (HEs) from commercially available/non-used MLs (ML-type, coating: PLS, Bioline (R); Hilite7000LT, X.ELLENCE (R); Nautilus, Balance (R); EOS, PH.I.S.I.O (R)) included static hemolysis and platelet adhesion/activation assays. Platelet activation of non-adherent platelets was identified after antibody (CD62P, PAC-1, CD61) and fibrinogen staining (flow cytometry). The surface coverage (%) of adherent platelets was quantified after F-actin filament-staining. Results: All materials were non-hemolytic and did not induce platelet activation. However, platelet adhesion (median (IQR)) depended on the type of surface coating of GFs made entirely of polymethylpentene. Both uncoated GFs (12 (7-19)%) and X.ELLENCE-coated GFs (Hilite-ML, 13 (8-19)%) showed a significantly higher surface coverage compared to Balance-coated GFs (Nautilus-ML, 3 (1-6)%), PH.I.S.I.O-coated GFs (EOS-ML, 2 (2-5)%) and Bioline-coated GFs (PLS-ML, 4 (1-8)%) (p < 0.001). HEs made of polyethyleneterephthalate (Hilite-ML, Nautilus-ML) that were coated with X.ELLENCE were covered with more platelets (5 (3-7)%) compared to Balance-coated HEs (3 (1-6)%), respectively (p = 0.029). Conclusions: In vitro testing disclosed fourfold higher platelet adhesion on X.ELLENCE-coated GFs (and HEs) from the Hilite-ML compared to other ECMO-materials. Additional hemocompatibility tests are necessary to assess the increased platelet adhesion on the materials from the Hilite-ML.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RESPIRATORY-DISTRESS-SYNDROME; SURFACE-COATINGS; ADHESION; HEPARIN; HEMOCOMPATIBILITY; COAGULATION; ECMO; platelet activation; platelet adhesion; hemolysis; thrombosis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 May 2026 09:16 |
| Last Modified: | 26 May 2026 09:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/65875 |
Actions (login required)
 |
View Item |
