Haile, Sophie-Marie and Gruber, Michael and Bollwein, Gabriele and Trabold, Benedikt (2025) Effect of Arginine Vasopressin on Human Neutrophil Function Under Physiological and Sepsis-Associated Conditions. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 26 (6): 2512. ISSN 1661-6596, 1422-0067
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This study examines how different concentrations of arginine vasopressin (AVP) and its preservative chlorobutanol (ClB) impact the immune functions of human polymorphonuclear neutrophils (PMNs), which are crucial in the immune response, particularly in sepsis. Using a model to simulate the physiological, sepsis-related, and therapeutic AVP levels in plasma, we analysed how AVP and ClB affect PMN activities, including reactive oxygen species (ROS) production, NETosis, antigen expression, and migration. PMNs were isolated from whole human blood and assessed using flow cytometry and live cell imaging. The results indicated that neither AVP nor ClB significantly affected PMN viability, antigen expression, NETosis, or ROS production in response to N-Formylmethionine-leucyl-phenylalanine, or fMLP, and tumour necrosis factor alpha. In the migration assays, concentration-dependent effects were observed. At physiological AVP levels, PMN migration showed no reduction, while the sepsis-associated AVP levels initially reduced migration before returning to the baseline or even increasing. The therapeutic AVP concentrations showed similar migration to that in the controls, while high concentrations progressively inhibited migration. ClB, regardless of its concentration, enhanced PMN migration. These findings suggest that AVP during sepsis may impair PMN migration, potentially contributing to tissue damage and systemic complications. This highlights AVP's role as a possible immune modulator in complex immune responses.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RESPIRATORY BURST; PLASMA OXYTOCIN; CHLOROBUTANOL; RECEPTOR; HORMONE; SYSTEM; CHEMOTAXIS; ACTIVATION; MIGRATION; BINDING; neutrophils; sepsis; arginine vasopressin; chlorobutanol; NETosis; ROS; migration; live cell imaging; antigen expression (CD11b, CD62L) |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Jun 2026 08:28 |
| Last Modified: | 02 Jun 2026 08:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/65905 |
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