Reichelt-Wurm, Simone and Knauss, Lena and Strasser, Bettina and Scharf, Mona and Holler, Kathrin and Eggenhofer, Elke and Kretz, Markus and Banas, Bernhard and Banas, Miriam C. (2025) Cell-type specific effects of the long non-coding RNA HIF1A-AS3 on HIF1A expression in kidney cells. SCIENTIFIC REPORTS, 15 (1): 27876. ISSN 2045-2322,
Full text not available from this repository. (Request a copy)Abstract
The hypoxia-inducible factor 1 alpha (Hif1 alpha) represents the master transcription factor coordinating cellular responses to oxygen depletion. With hundreds of target genes it plays a key role in numerous bio-medical conditions as well as neoplastic and non-cancerous diseases, which in turn requires a strict regulation. Long non-coding RNAs have the potential to virtually control every step of gene expression. We aimed to investigate the expression and role of HIF1A antisense lncRNAs HIF1A-AS1, AS2, and AS3 under hyperglycemic, hypoxic, or both conditions in three non-cancerous human renal cell types: HK-2 cells, primary RPTECs, and mesangial cells. We observed that HIF1A-AS2 and AS3 expression was upregulated under oxygen deprivation. Furthermore, knockdown (KD) of HIF1A-AS3 resulted in a significant reduction of HIF1A-AS2 and even more important of Hif1 alpha in HK-2 cells but not mesangial cells. While KD of HIF1A also had a diminishing effect on HIF1A-AS2 and AS3 RNA levels, KD of HIF1A-AS2 only affected HIF1A-AS3 but not HIF1A. Treating HK-2 cells with Actinomycin D revealed a high HIF1A-AS3 RNA stability. In conclusion, our data reveal a cell-type specific effect of HIF1A-AS3 on HIF1A RNA and protein expression which might allow the development of a cell-type specific HIF1A antagonist based on lncRNAs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LNCRNA HIF1A-AS1; HYPOXIA; CANCER; HIF-1-ALPHA; TRANSCRIPTION; ANGIOGENESIS; REPERFUSION; ACTIVATION; HIF1A; HIF1A-AS3; HIF1A-AS2; Hypoxia; Hyperglycemia; Oxidative stress |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Abteilung für Nephrologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Oct 2026 06:21 |
| Last Modified: | 13 Oct 2026 06:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/66188 |
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