Akamp, Tobias and Pohl, Sandra and Scholz, Konstantin J. and Sigl, Philipp and Rosendahl, Andreas and Woelflick, Melanie and Pielnhofer, Florian and Buchalla, Wolfgang and Widbiller, Matthias (2025) In-vitro-cytotoxicity of cariostatic agents based on fluorides and lanthanide salts in L-929 fibroblasts. CLINICAL ORAL INVESTIGATIONS, 29 (7): 366. ISSN 1432-6981, 1436-3771
Full text not available from this repository. (Request a copy)Abstract
ObjectivesFluoride-based cariostatic agents are commonly used in dental products and are generally considered safe. However, ongoing discussions about potential adverse effects are driving research into alternative agents, such as lanthanide salts. This study aims to evaluate the cytotoxic effects of different cariostatic agents, including fluoride compounds (NaF, Na2PO3F, NH4F) and lanthanide salts (Ce(NO3)3, CeCl3, Sm(NO3)3, SmCl3).Materials and methodsMouse fibroblasts (L-929) were cultured in Eagle's Minimum Essential Medium supplemented with 5% fetal bovine serum and 1% penicillin-streptomycin. Cell viability was assessed via MTT assay after 24 h of exposure to test compounds at concentrations of 0.0128, 0.064, 0.32, 1.6, 8, 40, 200 and 1000 mM, and lactate dehydrogenase (LDH) release was quantified to assess membrane integrity. The accumulation of reactive oxygen species (ROS) was determined after 24 h. Data were analyzed by non-parametric methods at a significance level of alpha = 0.05 (Mann-Whitney U and Kruskal-Wallis tests).ResultsCell viability decreased significantly for NaCl and NaNO3 at 200 mM, and for NaF, NH4F, Ce(NO3)3, CeCl3, Sm(NO3)3 and SmCl3 at 1.6 mM, falling below 70% of control (P <= 0.0178). Similarly, LDH assays indicated a significant incline in cytotoxicity at a concentration of 200 mM for NaCl, NaNO3 and Na2PO3F, and at 8 mM for NaF, NH4F and all lanthanide compounds (P <= 0.0016). ROS quantification showed that NaF, NH4F, CeCl3, Sm(NO3)3 and SmCl3 induced oxidative stress at 1.6 mM with statistical significance (P <= 0.0065).ConclusionsFluoride and lanthanide compounds exhibited similar in vitro biocompatibility, comparable to that of table salt.Clinical relevanceBoth fluoride- and lanthanide-based cariostatic agents appear to pose a low biological risk to surrounding oral tissues when used at appropriate doses in dental products.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SODIUM-FLUORIDE; CERIUM CHLORIDE; ENAMEL; TOXICITY; PLAQUE; DAMAGE; ROS; MONOFLUOROPHOSPHATE; PROLIFERATION; DENTIFRICES; Cariostatic agents; Toothpastes; Fluorides; Lanthanoid series elements; Cell survival; Cell death |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie Chemistry and Pharmacy > Institut für Anorganische Chemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Jun 2026 09:51 |
| Last Modified: | 16 Jun 2026 09:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/66237 |
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