Penzkofer, Alfons and Silapetere, Arita and Hegemann, Peter (2025) Reinvestigation of Absorption Spectroscopic Thermal Dynamics of Archaerhodopsin 3 Based Voltage Sensor QuasAr1. BIOENGINEERING-BASEL, 12 (12): 1293. ISSN 2306-5354
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The long-time absorption spectroscopic development of the genetically encoded microbial rhodopsin fluorescent voltage indicator QuasAr1 at room temperature in the dark was reinvestigated, mainly theoretically. The data analysis indicates protein aggregation within one day to some ten-nanometer sized Mie scattering particles. The absorption coefficient spectra can be deduced from measured attenuation coefficient spectra by scattering contribution subtraction. The initially present protonated retinal Schiff base (PRSB) Ret_580 isomerized and then deprotonated to neutral retinal Schiff base (RSB). One part of Ret_580, Ret_580I, (fraction 43%), isomerized moderately fast to Ret_500 which then deprotonated to neutral retinal Schiff base Ret_405 (time constant approximate to 1000 h). The other part of Ret_580, Ret_580II, (fraction 57%), isomerized slowly to Ret_460 which deprotonated to Ret_340 (time constant approximate to 400 h). The dynamics are described by a differential equation system which is solved numerically. Reaction parameters are determined by fitting the simulations to the experimental results.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | QuasAr1; Archaerhodopsin; fluorescent voltage sensor; absorption spectroscopic characterization; protein aggregation; Rayleigh and Mie scattering; numerical simulation of thermal dynamics; ground-state isomerization; ground-state deprotonation |
| Subjects: | 500 Science > 530 Physics |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Apr 2026 04:42 |
| Last Modified: | 22 Apr 2026 04:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/66393 |
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