Labudda, Kristin and Norahan, Mohamad Javad and Huebner, Lisa-Marie and Althoff, Philipp and Gerwert, Klaus and Luebben, Mathias and Rudack, Till and Koetting, Carsten (2025) A second photoactivatable state of the anion-conducting channelrhodopsin GtACR1 empowers persistent activity. COMMUNICATIONS BIOLOGY, 8 (1): 1183. ISSN 2399-3642
Full text not available from this repository. (Request a copy)Abstract
Optogenetics is a method to regulate cells, tissues and organisms using light. It is applied to study neurons and to develop diagnostic and therapeutic tools for neuron-related diseases. The cation-conducting channelrhodopsin ChR2 triggers photoinduced depolarization of neuronal cells but generates lower ion currents due to the syn-pathway of its branched photocycle. In contrast, the homologous anion-conducting ACR1 from Guillardia theta (GtACR1), exhibits high photocurrents. Here, we investigate the mechanistic cause for the observed high photocurrents in GtACR1 using FTIR spectroscopy. Unexpectedly, we discovered that the O intermediate of GtACR1 is photoactivable, allowing for fast and efficient channel reopening. Our vibrational spectra show a photocyclic reaction sequence after O excitation similar to the ground state photocycle but with slightly altered channel conformation and protonation states. Our results provide deeper insights into the gating mechanism of channelrhodopsins and pave the way to advance the development of optimized optogenetic tools in future.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MICROBIAL RHODOPSINS; RETINAL CHROMOPHORE; OPTOGENETIC CONTROL; WATER-MOLECULES; PROTON-TRANSFER; LIGHT; MEMBRANE; PROTEIN; BACTERIORHODOPSIN; CHANNEL |
| Subjects: | 500 Science > 530 Physics 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Till Rudack Regensburg Center for UltrafastNanoscopy (RUN) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Sep 2026 08:13 |
| Last Modified: | 23 Sep 2026 08:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/66442 |
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