Grotz, Barbara V. and Rogalla von Bieberstein, Klara and Wongkaew, Nongnoot and Duerkop, Axel and Frey, Margaret W. and Baeumner, Antje J. (2025) Engineered Nanofiber-Hydrogel Systems for Colorimetric Lactate Sensing from Breath. ACS APPLIED MATERIALS & INTERFACES, 17 (46). pp. 63009-63019. ISSN 1944-8244, 1944-8252
Full text not available from this repository. (Request a copy)Abstract
Current methods for detecting chronic airway inflammation, such as asthma, rely on complex procedures and specialized clinicians. Taking advantage of inherent nanomaterial properties and their chemical design flexibility, nanofibers were designed and integrated with enzyme entrapping hydrogels. This composition offers noninvasive sample collection followed by simple colorimetric detection. Specifically, nanofibers were made from positively charged nylon-poly(allylamine hydrochloride). They were optimized with respect to mat thickness, additive content, and lactate capture efficiency. The nanofibers could efficiently bind lactate through electrostatic interaction, correlating the resulting amount on the nanofiber mat to the concentration in breath aerosols. Detection was subsequently accomplished through a standard lactate oxidase, horseradish peroxidase assay with 3,3 ',5,5 '-tetramethylbenzidine colorimetric detection. The optimized nanofibers outperformed other polymeric nanofibers, face mask material, and filter paper regarding analyte capture and breathability due to the surface chemistry chosen and the high surface area afforded through the nanofiber mats. For lactate quantification directly on the mask, lactate oxidase was immobilized on the nanofiber mat via a hydrogel, ensuring long-term storage stability. Simple visual detection was achieved providing limits of detection of 5 mu mol<middle dot>L-1 (in solution) and 20 mu mol<middle dot>L-1 (hydrogel-based system) and a dynamic range that covers lactate concentrations found in breath, i.e., 5 to 150 mu mol<middle dot>L-1. This platform technology offers a promising solution for point-of-care diagnostics, contributing to remote healthcare, telemedicine, and simplified diagnostics in airway inflammation management.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CAPILLARY-ELECTROPHORESIS; MOLECULAR-WEIGHT; BIOSENSOR; CONDENSATE; LUNG; OPTIMIZATION; FILTRATION; EFFICIENCY; HRP; point-of-care diagnostics; wearable system; breath analysis; nanofibers; noninvasive sampling; colorimetric lactate detection |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Mar 2026 04:26 |
| Last Modified: | 31 Mar 2026 04:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/66587 |
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