Rupp, M. and Mannala, G. K. and Walter, N. and Mendelsohn, D. and Roupie, C. and Ladam, G. and Labat, B. and Wong, R. M. Y. and Cheung, W. H. and Brochhausen, Christoph and Alt, Volker and Riool, M. and Thebault, P. (2025) Nisin-enriched coatings on titanium implants preventStaphylococcus aureusbiofilm formation: the Galleria mellonella model as a testing platform. EUROPEAN CELLS & MATERIALS, 51. pp. 136-144. ISSN 1473-2262
Full text not available from this repository. (Request a copy)Abstract
Background: Bone and joint infections pose significant clinical challenges, often leading to severe complications and substantial healthcare costs. Traditional antibiotic therapies are becoming increasingly ineffective due to rising antibiotic resistance and the biofilm-forming ability of bacteria such as Staphylococcus aureus (S. aureus). Antimicrobial coatings offer a promising approach for the prevention and treatment of implant-associated and bone infections. Following the demonstrated in vitro efficacy of nisin, a naturally occurring antimicrobial peptide, in preventing S. aureus biofilm formation, this study investigates the in vivo potential of a nisin-enriched coating to prevent biofilm-related infections using the Galleria mellonella larva haematogenous implant infection model. Methods: Methicillin-sensitive S. aureus (MSSA) EDCC 5055 was used to infect larvae implanted with nisin-coated titanium Kirschner wires (K-wires). Survival rates and bacterial loads on both the K-wires and in larval tissue were analysed. Biofilm formation on K-wires was further analysed using scanning electron microscopy. Results: The results showed that nisin-coated K-wires significantly improved larval survival and reduced bacterial burden compared to control groups. Scanning electron microscopy confirmed the absence of biofilm formation on nisin-coated K-wires. Conclusions: These findings suggest that nisin-enriched coatings could be a viable strategy for preventing bone and joint infections. Additionally, this study demonstrates the feasibility of testing implant coatings in a cost-effective and ethically sound alternative in vivo model. Further evaluation and testing of the nisin-enhanced coating in vertebrate animal implant infection models is warranted.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Nisin; titanium implant; Staphylococcus aureus; Galleria mellonella |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 May 2026 05:48 |
| Last Modified: | 12 May 2026 05:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/66690 |
Actions (login required)
 |
View Item |
