Simon, Daniel and Horkovics-Kovats, Gabriel Stefan and Xiang, Yuchen and Battle, Ronan A. and Wang, Yu and Abda, Julia and Papanastasiou, Dimitris and Stavrakaki, Stefania Maneta and Ho, Hui-Yu and Wang, Haixing and Schaeffer, Richard and Karancsi, Tamas and Mroz, Anna and Pap, Istvan and Lagache, Laurine and Balog, Julia and Fournier, Isabelle and Murray, Robert T. and Bunch, Josephine and Takats, Zoltan (2025) Subcellular-Resolution Molecular Pathology by Laser Ablation-Rapid Evaporative Ionization Mass Spectrometry. ANALYTICAL CHEMISTRY, 97 (32). pp. 17433-17443. ISSN 0003-2700, 1520-6882
Full text not available from this repository. (Request a copy)Abstract
This work demonstrates the combination of ambient laser ablation (LA) with in-source surface-induced declustering, originally developed for rapid evaporative ionization mass spectrometry (REIMS). This combination, termed laser ablation REIMS (LA-REIMS), provides sensitivity, spatial resolution, and chemical coverage comparable to matrix-assisted laser desoprtion ionization (MALDI) but without the requirement for matrix deposition. The atmospheric pressure interface setup was subjected to detailed characterization with regard to geometric and thermal parameters augmented by in-silico flow modeling. The resulting platform was tested using aerosol formed by the infrared laser ablation of tissues. Three different laser systems were successfully employed for ambient mass spectrometric imaging: a carbon dioxide laser (lambda = 10.6 mu m, tau L = similar to 100 mu s), an optical parametric oscillator (OPO; lambda = 2.94 mu m, tau L = 8 ns), and an optical parametric amplifier (OPA; lambda = 3.0 mu m, tau L = similar to 30 ps). Single-cell imaging was achieved using the high-resolving capabilities of the OPA systems, and metabolites and lipids ranging from amino acids through carbohydrates and nuclear bases to complex glycolipids were successfully detected. The technique was also tested as a platform for MS-guided surgery, raising the possibility of using a single technique for generating histological and in vivo data.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ELECTROSPRAY-IONIZATION; ATMOSPHERIC-PRESSURE; IN-VIVO; DESORPTION IONIZATION; IDENTIFICATION; CANCER; PERFORMANCE; MECHANISMS; IONS; SITU; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Sep 2026 08:22 |
| Last Modified: | 15 Sep 2026 08:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/67147 |
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