Li, Shushan and Guo, Pei and Wang, Li and Zhang, Yi and Li, Sanhua and Liu, Chang and Dang, Haibin and Yin, Li and Grassel, Susanne (2025) miR-127-5p-enriched extracellular vesicles modulate the Hippo pathway to counteract steroid-induced osteonecrosis pathology of the femoral head. FREE RADICAL BIOLOGY AND MEDICINE, 238. pp. 90-104. ISSN 0891-5849, 1873-4596
Full text not available from this repository. (Request a copy)Abstract
Steroid-induced osteonecrosis of the femoral head (SONFH) is a debilitating condition characterized by bone tissue death and joint dysfunction. This study explored the role of miR-127-5p in SONFH-human bone marrow derived stromal cells (hBMSCs), focusing on its impact on the Hippo signaling pathway and the therapeutic potential of miR-127-5p-enriched extracellular vesicles (EVs). Quantitative real-time PCR revealed that miR-1275p is significantly downregulated in SONFH-hBMSCs, leading to the upregulation of LATS2 and the suppression of YAP/TAZ-TEAD activity, which are crucial for osteogenesis. EVs derived from SONFH-hBMSCs further inhibited osteogenic differentiation and promoted adipogenesis. However, miR-127-5p-enriched EVs reversed these effects, restoring osteogenic differentiation, improving bone microarchitecture, and enhancing gait performance in SONFH models. These findings suggest that miR-127-5p downregulation plays a critical role in SONFH progression and that miR-127-5p-enriched EVs may offer a promising therapeutic strategy to treat SONFH by promoting bone regeneration and improving functional outcomes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESENCHYMAL STEM-CELLS; Steroid-induced osteonecrosis of the femoral; head; EVs; hBMSC; Osteogenic differentiation; miRNA; Hippo pathway |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 May 2026 11:32 |
| Last Modified: | 26 May 2026 11:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/67430 |
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