Wustemann, Till and Madzharova, Elizabeta and Wietecha, Mateusz S. and Ghyselinck, Norbert B. and Höring, Marcus and Liebisch, Gerhard and Zamboni, Nicola and Keller, Ulrich Auf Dem and Werner, Sabine (2025) A multiomics analysis identifies retinol metabolism in fibroblasts as a key pathway in wound healing. JCI INSIGHT, 10 (22): e194188. ISSN , 2379-3708
Full text not available from this repository. (Request a copy)Abstract
Impaired wound healing poses a major and increasingly frequent health problem. Among the key players in the healing process are fibroblasts, but their metabolic profile in healing wounds is largely unknown. Using a combination of transcriptomics, targeted proteomics, and metabolomics, we identified retinol metabolism as a top regulated pathway in wound fibroblasts. This is functionally relevant, since even a mild retinol deficiency caused a delay in wound closure and reepithelialization, which mainly resulted from misdirected keratinocyte migration on the new granulation tissue. Quantitative proteomics identified integrin subunit alpha 11 as a less abundant protein in wounds of mice subjected to a retinol-deficient diet. Reduced levels of this fibroblast-specific protein likely altered the granulation tissue matrix, which in turn affected reepithelialization. These results provide a comprehensive overview of the transcriptome, proteome, and metabolome of wound fibroblasts and identify retinol metabolism in fibroblasts as a key regulator of tissue repair.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM MASS-SPECTROMETRY; HIGH-THROUGHPUT QUANTIFICATION; MATTHEW-WOOD-SYNDROME; VITAMIN-A; DERMAL FIBROBLASTS; GRANULATION-TISSUE; CELLULAR-EXTRACTS; TOPICAL TRETINOIN; LIPID EXTRACTION; RECEPTOR; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 May 2026 06:23 |
| Last Modified: | 19 May 2026 06:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/67455 |
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