Englert, Ludwig and Perleberg, Bjarne and Tayal, Shilpa and Wang, Bingzhi and Huang, Yishu and Mohammed, Azeem and He, Hailong and Stiel, Andre C. and Ntziachristos, Vasilis (2025) Assessment of performance characteristics of photoswitchable bacteriophytochromes at different depths using optoacoustic mesoscopy. OPTICS EXPRESS, 33 (16). pp. 32943-32954. ISSN 1094-4087,
Full text not available from this repository. (Request a copy)Abstract
Photoswitchable proteins have so far been barely investigated in the field of optoacoustic (OptA) mesoscopy. In particular, the unmixing performance and achievable depth with photoswitchable bacteriophytochromes in an OptA mesoscopy setup with a single-element detector remain uncharted. Therefore, this study investigates the switching kinetics and unmixing performance at two wavelengths of photoswitchable bacteriophytochromes expressed by E. coli cells in an OptA mesoscopy set up with a single-element spherically focused 25 MHz piezoelectric detector at different depths using a light scattering phantom. We found that the signal-to-noise ratio dropped by approximately 5 dB/mm as a consequence of declining detector sensitivity, attenuation of the acoustic wave, and decreasing fluence. This limited unmixing with the current system parameters to a depth of 2-4 millimeters. The analysis of the switching kinetics indicates that an increased number of laser pulses was needed to switch the protein between the ON-and OFF states as depth increases. Based on these analyzed switching kinetics, recommendations can be made regarding the pulsing schemes needed at different depths. The knowledge gained on the required pulsing schemes for successful protein switching, unmixing performance, and achievable depth can be leveraged in future ex-vivo/in-vivo studies using photoswitchable proteins combined with OptA mesoscopy. Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHOTOACOUSTIC TOMOGRAPHY; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 May 2026 12:07 |
| Last Modified: | 05 May 2026 12:07 |
| URI: | https://pred.uni-regensburg.de/id/eprint/67520 |
Actions (login required)
 |
View Item |
