Zourelidou, Melina and Absmanner, Birgit and Weller, Benjamin and Barbosa, Ines C. R. and Willige, Bjoern C. and Fastner, Astrid and Streit, Verena and Port, Sarah and Colcombet, Jean and van Bentem, Sergio de la Fuente and Hirt, Heribert and Kuster, Bernhard and Schulze, Waltraud X. and Hammes, Ulrich Z. and Schwechheimer, Claus (2014) Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID. ELIFE, 3: e02860. ISSN 2050-084X,
Full text not available from this repository.Abstract
The development and morphology of vascular plants is critically determined by synthesis and proper distribution of the phytohormone auxin. The directed cell-to-cell distribution of auxin is achieved through a system of auxin influx and efflux transporters. PIN-FORMED (PIN) proteins are proposed auxin efflux transporters, and auxin fluxes can seemingly be predicted based on the - in many cells - asymmetric plasma membrane distribution of PINs. Here, we show in a heterologous Xenopus oocyte system as well as in Arabidopsis thaliana inflorescence stems that PIN-mediated auxin transport is directly activated by D6 PROTEIN KINASE (D6PK) and PINOID (PID)/WAG kinases of the Arabidopsis AGCVIII kinase family. At the same time, we reveal that D6PKs and PID have differential phosphosite preferences. Our study suggests that PIN activation by protein kinases is a crucial component of auxin transport control that must be taken into account to understand auxin distribution within the plant.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARABIDOPSIS-THALIANA; TRANSPORT; GROWTH; FAMILY; PHOSPHORYLATION; ENDOCYTOSIS; PHYLLOTAXIS; INHIBITION; EXPRESSION; CARRIERS; Arabidopsis; AGC kinases; auxin; auxin efflux; auxin transport; protein kinases; plant |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Oct 2019 07:21 |
| Last Modified: | 28 Oct 2019 07:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10016 |
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