Kratzer, Alexander and Liebchen, Uwe and Schleibinger, Michael and Kees, Martin G. and Kees, Frieder (2014) Determination of free vancomycin, ceftriaxone, cefazolin and ertapenem in plasma by ultrafiltration: Impact of experimental conditions. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 961. pp. 97-102. ISSN 1570-0232, 1873-376X
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Ultrafiltration is a rapid and convenient method to determine the free concentrations of drugs. In the present work, we aimed to develop an ultrafiltration method which is appropriate for routine determination of the free fraction of vancomycin and highly protein bound beta-lactams such as ertapenem, ceftriaxone and cefazolin in plasma from intensive care unit patients. Different filter types and experimental conditions (molecular weight cut-off, centrifugal force and time, pH, temperature) were evaluated and found to have influence on the result. In the final protocol, serum or plasma was buffered to pH 7.4-7.5, ultrafiltered at 1000 x g at 37 degrees C for 20 min using Nanosep Omega 10 K filters and subsequently analysed for the antibiotics by RP-HPLC with UV detection. The data from our investigation suggest to aim physiological conditions, i.e. 37 degrees C and pH 7.4, and low to moderate relative centrifugal forces in order to get reliable results. With regard to the chromatographic separation, modulation of the pH in the range of 2.5-7.0 allows to determine several beta-lactams isocratically and/or to avoid interferences by co-administered drugs. (C) 2014 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PERFORMANCE LIQUID-CHROMATOGRAPHY; BETA-LACTAM ANTIBIOTICS; CRITICALLY-ILL PATIENTS; CARE-UNIT PATIENTS; PROTEIN-BINDING; ULTRAVIOLET DETECTION; EQUILIBRIUM DIALYSIS; BIOLOGICAL-FLUIDS; HUMAN-SERUM; PHARMACOKINETICS; Protein binding; Beta-lactam; pH; Temperature; Relative centrifugal force; HPLC |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Chemistry and Pharmacy > Institute of Pharmacy |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Oct 2019 08:32 |
| Last Modified: | 28 Oct 2019 08:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10021 |
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