Lirio, Stephen B. and Macabeo, Allan Patrick G. and Paragas, Erickson M. and Knorn, Matthias and Kohls, Paul and Franzblau, Scott G. and Wang, Yuehong and Aguinaldo, Ma. Alicia M. (2014) Antitubercular constituents from Premna odorata Blanco. JOURNAL OF ETHNOPHARMACOLOGY, 154 (2). pp. 471-474. ISSN 0378-8741,
Full text not available from this repository. (Request a copy)Abstract
Ethnopharmacological relevance: Premna odorata Blanco (Lamiaceae) is a medicinal plant traditionally used in Albay Province, in southeastern Luzon, Philippines to treat tuberculosis. This study aimed to determine the antitubercular property of the crude extract and sub-extracts of the leaves, and to isolate the bioactive principles from the active fractions. Materials and methods: Through extraction, solvent polarity-based fractionation and silica gel chromatography purification of the DCM sub-extract, compound mixtures from the bioactive fractions were isolated and screened for their in vitro antimycobacterial activity against Mycobacterium tuberculosis H(37)Rv using the colorimetric Microplate Alamar Blue assay (MABA). Results: The crude methanolic extract and sub-extracts showed poor inhibitory activity against Mycobacterium tuberculosis H(37)Rv (MIC >= 128 mu g/mL). However, increased inhibitory potency was observed for fractions eluted from the DCM sub-extract (MIC=54 to 120 mu g/mL). Further purification of the most active fraction (MIC=54 mu g/mL) led to the isolation of a 1-heneicosyl formate (1), 4:1 mixture of p-sitosterol (2), stigmasterol (3) and diosmetin (4), which were identified through GC MS analysis (with dereplication) and NMR experiments. The MIC of compound 1 was 8 mu g/mL. Conclusions: The results of this study provide scientific basis for the traditional use of Premna odorata as treatment for tuberculosis. (C) 2014 Published by Elsevier Ireland Ltd.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MYCOBACTERIUM-TUBERCULOSIS; ANTIBACTERIAL ACTIVITY; IN-VITRO; ALKALOIDS; ASSAY; Premna odorata; Antitubercular; Tuberculosis; 1-heneicosyl formate; Diosmetin |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Sep 2019 11:26 |
| Last Modified: | 16 Sep 2019 11:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10027 |
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