Nass, Bastian and Poell, Uwe and Langer, Julian David and Kreuter, Lydia and Kueper, Ulf and Flechsler, Jennifer and Heimerl, Thomas and Rachel, Reinhard and Huber, Harald and Kletzin, Arnulf (2014) Three multihaem cytochromes c from the hyperthermophilic archaeon Ignicoccus hospitalis: purification, properties and localization. MICROBIOLOGY-SGM, 160. pp. 1278-1289. ISSN 1350-0872,
Full text not available from this repository. (Request a copy)Abstract
Three different multihaem cytochromes c were purified from cell extracts of the hyperthermophilic archaeon lgnicoccus hospitalis. One tetrahaem cytochrome, locus tag designation Igni_0530, was purified from membrane fractions together with the iron-sulfur protein Igni_0529. Two octahaem cytochromes, Igni_0955 and Igni_1359, were purified from soluble fractions but were also present in the membrane fraction. N-terminal sequencing showed that three of the four proteins had their signal peptides cleaved off, while results were ambiguous for Igni_0955. In contrast, mass spectrometry of Igni_0955 and Igni_1359 resulted in single mass peaks including the signal sequences and eight haems per subunit and so both forms might be present in the cell. Igni_0955 and Igni_1359 belong to the hydroxylamine dehydrogenase (HAO) family (29 % mutual identity). HAO or reductase activities with inorganic sulfur compounds were not detected. Igni_0955 was reduced by enriched I. hospitalis hydrogenase at a specific activity of 243 nmol min(-1) (mg hydrogenase)(-1) while activity was non-existent for Igni_0530 and low for Igni_1359. Immuno-electron microscopy of ultra-thin sections showed that Igni_0955 and Igni_1359 are located in both I. hospitalis membranes and also in the intermembrane compartment. We concluded that these cytochromes might function as electron shuttles between the hydrogenase in the outer cellular membrane and cellular reductases, whereas Igni_0530 might be part of the sulfur-reducing mechanism.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ELECTRON-TRANSPORT; HALOBACTERIUM-SALINARIUM; NANOARCHAEUM-EQUITANS; ANAEROBIC RESPIRATION; POLYACRYLAMIDE-GELS; COLLOIDAL SULFUR; SYSTEM-I; MEMBRANE; PROTEINS; BACTERIA; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Sep 2019 07:27 |
| Last Modified: | 18 Sep 2019 07:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10065 |
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