Pleiser, Sandra and Al Banchaabouchi, Mumna and Samol-Wolf, Annette and Farley, Dominika and Welz, Tobias and Wellbourne-Wood, Joel and Gehring, Isabell and Linkner, Joern and Faix, Jan and Riemenschneider, Markus J. and Dietrich, Susanne and Kerkhoff, Eugen (2014) Enhanced fear expression in Spir-1 actin organizer mutant mice. EUROPEAN JOURNAL OF CELL BIOLOGY, 93 (5-6). pp. 225-237. ISSN 0171-9335, 1618-1298
Full text not available from this repository. (Request a copy)Abstract
Spir proteins nucleate actin filaments at vesicle membranes and facilitate intracellular transport processes. The mammalian genome encodes two Spit proteins, namely Spir-1 and Spir-2. While the mouse spir-2 gene has a rather broad expression pattern, high levels of spir-1 expression are restricted to the nervous system, oocytes, and testis. Spir-1 mutant mice generated by a gene trap method have been employed to address Spir-1 function during mouse development and in adult mouse tissues, with a specific emphasis on viability, reproduction, and the nervous system. The gene trap cassette disrupts Spir-1 expression between the N-terminal KIND domain and the WH2 domain cluster. Spir-1 mutant mice are viable and were born in a Mendelian ratio. In accordance with the redundant function of Spir-1 and Spir2 in oocyte maturation, spir-1 mutant mice are fertile. The overall brain anatomy of spir-1 mutant mice is not altered and visual and motor functions of the mice remain normal. Microscopic analysis shows a slight reduction in the number of dendritic spines on cortical neurons. Detailed behavioral studies of the spir-1 mutant mice, however, unveiled a very specific and highly significant phenotype in terms of fear learning in male mice. In contextual and cued fear conditioning experiments the male spir-1 mutant mice display increased fear memory when compared to their control littermates. Our data point toward a particular function of the vesicle associated Spir-1 actin organizer in neuronal circuits determining fear behavior. (C) 2013 Elsevier GmbH. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SYNAPTIC PLASTICITY; VESICLE TRANSPORT; NUCLEATION FACTOR; CONTEXTUAL FEAR; MEMORY; HIPPOCAMPUS; FORMIN-2; INHIBITION; CAPPUCCINO; ENDOSOMES; Actin; Formin; Spir-1; Fmn-2; Mouse genetics; Nervous system; Emotional learning; Fear conditioning |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Sep 2019 12:21 |
| Last Modified: | 23 Sep 2019 12:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10203 |
Actions (login required)
 |
View Item |
