Proff, Peter and Reicheneder, Claudia and Faltermeier, Andreas and Kubein-Meesenburg, Dietmar and Roemer, Piero (2014) Effects of mechanical and bacterial stressors on cytokine and growth-factor expression in periodontal ligament cells. JOURNAL OF OROFACIAL ORTHOPEDICS-FORTSCHRITTE DER KIEFERORTHOPADIE, 75 (3). pp. 191-202. ISSN 1434-5293, 1615-6714
Full text not available from this repository. (Request a copy)Abstract
The goal of the study was to examine the effects of a mechanical (orthodontic force simulation by static compressive loading) and a bacterial (endotoxins from a heat-inactivated gram-negative periodontal pathogen) stressor on the expression patterns of factors that are key to regulating osteoclastogenesis and bone remodeling. Three experimental groups were formed with fifth-passage periodontal ligament (PDL) fibroblasts treated by the static application of compressive force (2 g/cm(2)), heat-inactivated aggregatibacter actinomycetemcomitans (1 x 10(7) cells), or both of these stressors combined. Real-time polymerase chain reaction (RT-PCR) was used to study gene expression of IL-6, IL-8, COX-2, IGF-1, VEGF, and MMP-13 in the 3 groups. Protein levels of COX-2, prostaglandin E2 (PGE(2)), and IL-8 production were quantified using immunoblotting and enzyme-linked immunosorbent assay (ELISA). The mechanical stressor upregulated the genes of COX-2, IL-8, IGF-1, and MMP-13 in PDL fibroblasts and the bacterial stressor upregulated IL-6, IL-8, COX-2 and MMP-13. Both stressors in combination upregulated VEGF and caused COX-2 gene expression to increase further; the latter effect was also detected at the protein level and indirectly via the enhanced production of PGE(2). We noted that the posttranscriptional regulation of IL-8 was induced by the mechanical stressor and influenced by PGE(2). While mechanical-stressor application increased the gene expression of COX-2, IL-8, and VEGF in the presence of the bacterial stressor, IL-8 production was posttranscriptionally regulated by the mechanical stressor, whereas COX-2 expression correlated with enhanced production of the inflammatory tissue hormone PGE(2), which exerted a suppressive effect on endotoxin-induced IL-8 production.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ORTHODONTIC TOOTH MOVEMENT; PROSTAGLANDIN E-2; SUBGINGIVAL MICROBIOTA; COMPRESSIVE FORCE; CREVICULAR FLUID; GENE-EXPRESSION; BONE-RESORPTION; ROOT RESORPTION; PDL CELLS; KAPPA-B; Aggregatibacter actinomycetemcomitans; Orthodontic tooth movement; Cyclooxygenase-2; Periodontal ligament fibroblasts; Gene expression |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Nov 2019 09:50 |
| Last Modified: | 11 Nov 2019 09:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10204 |
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