Treiber, Thomas and Treiber, Nora and Plessmann, Uwe and Harlander, Simone and Daiss, Julia-Lisa and Eichner, Norbert and Lehmann, Gerhard and Schall, Kevin and Urlaub, Henning and Meister, Gunter (2017) A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis. MOLECULAR CELL, 66 (2). 270-+. ISSN 1097-2765, 1097-4164
Full text not available from this repository. (Request a copy)Abstract
During microRNA (miRNA) biogenesis, two endonucleolytic reactions convert stem-loop-structured precursors into mature miRNAs. These processing steps can be posttranscriptionally regulated by RNA-binding proteins (RBPs). Here, we have used a proteomics-based pull-down approach to map and characterize the interactome of a multitude of pre-miRNAs. We identify similar to 180 RBPs that interact specifically with distinct pre-miRNAs. For functional validation, we combined RNAi and CRISPR/Cas-mediated knockout experiments to analyze RBP-dependent changes in miRNA levels. Indeed, a large number of the investigated candidates, including splicing factors and other mRNA processing proteins, have effects on miRNA processing. As an example, we show that TRIM71/LIN41 is a potent regulator of miR-29a processing and its inactivation directly affects miR-29a targets. We provide an extended database of RBPs that interact with pre-miRNAs in extracts of different cell types, highlighting a widespread layer of co- and posttranscriptional regulation of miRNA biogenesis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SEQUENCE DETERMINANTS; LIN28-LET-7 PATHWAY; SECONDARY STRUCTURE; GENE-REGULATION; PRE-MICRORNA; HNRNP A1; URIDYLATION; RECOGNITION; DROSOPHILA; TARGET; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:10 |
| Last Modified: | 28 Feb 2019 10:45 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1023 |
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