Winkler, Cornelia and Schaefer, Lina and Felthaus, Oliver and Allerdings, Juri and Hahnel, Sebastian and Behr, Michael and Buergers, Ralf (2014) The bacterial adhesion on and the cytotoxicity of various dental cements used for implant-supported fixed restorations. ACTA ODONTOLOGICA SCANDINAVICA, 72 (4). pp. 241-250. ISSN 0001-6357, 1502-3850
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Objective. Bacterial adhesion on and cytotoxicity of eight luting agents used for implant-supported restorations were investigated. Materials and method. Surface roughness (Ra), surface free energy (SFE) values and three-dimensional images by atomic-force microscopy of circular specimens were determined. Bacterial suspensions of Streptococcus sanguinis and Streptococcus epidermidis were incubated at 37 degrees C for 2 h. Adhering bacteria were examined with fluorescence dye CytoX-Violet, stained with 4', 6-diamidino-2-phenylindole (DAPI) and visualized by fluorescence-microscopy. Cytotoxicity-testing was done with WST-1-tests (water soluble tetrazolium). No significant differences, neither with regard to Ra nor regarding SFE were determined. Results. Adherence of S. sanguinis was less on titanium, TempBondNE and TempBond. TempBond, TempBondNE, RelyX Unicem and Implantlink Semi Classic presented low amounts of S. epidermidis. WST-testing showed high cytotoxic potential of Harvard, Aqualox, TempBondNE and TempBond. No combination of low adherent bacteria with low cytotoxicity was found. Conclusion. From a biological in-vitro perspective, none of the cements may be recommended for implant-supported restorations.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GLASS-IONOMER CEMENTS; SURFACE-FREE-ENERGY; OSSEOINTEGRATED ORAL IMPLANTS; IN-VITRO CYTOTOXICITY; STREPTOCOCCUS-MUTANS; BIOFILM FORMATION; PLAQUE-FORMATION; TITANIUM ABUTMENTS; SUBGINGIVAL PLAQUE; MICROBIAL ADHESION; Streptococcus sanguinis; streptococcus epidermidis; dental implant; surface roughness; surface free energy; dental cement |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnärztliche Prothetik Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Plastische-, Hand- und Wiederherstellungschirurgie Physics > Institute of Experimental and Applied Physics |
| Depositing User: | Petra Gürster |
| Date Deposited: | 24 Jul 2020 07:41 |
| Last Modified: | 24 Jul 2020 07:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10257 |
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